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Anthropogenic carbon dioxide (CO2) release in the atmosphere from fossil fuel combustion has inspired scientists to study CO2 to fuel conversion. Oxygenic phototrophs such as cyanobacteria have been used to produce biofuels using CO2. However, oxygen generation during oxygenic photosynthesis affects biofuel production efficiency. To produce n-butanol (biofuel) from CO2, here we introduced an n-butanol biosynthesis pathway into an anoxygenic (non-oxygen evolving) photoautotroph, Rhodopseudomonas palustris TIE-1 (TIE-1). Using different carbon, nitrogen, and electron sources, we achieved n-butanol production in wild-type TIE-1 and mutants lacking electron-consuming (nitrogen-fixing) or acetyl-CoA-consuming (polyhydroxybutyrate and glycogen synthesis) pathways. The mutant lacking the nitrogen-fixing pathway produced highest n-butanol. Coupled with novel hybrid bioelectrochemical platforms, this mutant produced nbutanol using CO2, solar panel-generated electricity, and light, with high electrical energy conversion efficiency. Overall, this approach showcases TIE-1 as an attractive microbial chassis for carbon-neutral n-butanol bioproduction using sustainable, renewable, and abundant resources. 

This Letter reports the first measurement of the oscillation amplitude and frequency of reactor antineutrinos at Daya Bay via neutron capture on hydrogen using 1958 days of data. With over 3.6 million signal candidates, an optimized candidate selection, improved treatment of backgrounds and efficiencies, refined energy calibration, and an energy response model for the capture-on-hydrogen sensitive region, the relative ${\overline{\nu }}_e$rates and energy spectra variation among the near and far detectors gives ${\sin }^{2}2{\theta }_{13}=0.0759_{-0.0049}^{+0.0050}$and $\mathrm{\Delta }{m}_{32}^2=\left(2.72_{-0.15}^{+0.14}\right)\times {10}^{-3}{\mathrm{eV}}^2$assuming the normal neutrino mass ordering, and $\mathrm{\Delta }{m}_{32}^2=(-2.83_{-0.14}^{+0.15})\times {10}^{-3}{\mathrm{eV}}^2$for the inverted neutrino mass ordering. This estimate of ${\sin }^{2}2{\theta }_{13}$is consistent with and essentially independent from the one obtained using the capture-on-gadolinium sample at Daya Bay. The combination of these two results yields ${\sin }^{2}2{\theta }_{13}=0.0833\pm 0.0022$, which represents an 8% relative improvement in precision regarding the Daya Bay full 3158-day capture-on-gadolinium result. Published by the American Physical Society2024